Ferreira, Tiago and Wayne Rasband, The Image J User Guide, /ij/docs/user-guide.
and Eliceiri, K.W., BMC Bioinformatics, 18(1), 529 Terdapat dua metode modifikasi yaitu dengan cara memproteksi gugus hidroksil. T., Schindelin, J., Hiner, M.C., Dezonia, B.E., Walter, A.E., Arena, E.T. Lutfi, Wiwit Alwi, Ferli Trinoveldi, Iman Rahayu, Lena Rahmidar, Kancono Warsito, Procedia Environ. Tie-xin, Tang and Wu Hong, An Image Analysis System for Thin-Layer Chromatography Quantification and Its Validation, J. Sirait, Midian, Penuntun Fitokimia dalam Farmasi, 2007, Penerbit ITB, Bandung. Elumalai, Merina Benny, and Benny Antony, J. Wonorahardjo, Surjani, Metode-Metode Pemisahan Kimia, 2016, Indeks Akademia, Jakarta Nihayati, Ellis, Tatik Wardiyati, Rurini Retnowati, and Soemarno, Agrivita J. Departemen Kesehatan Republik Indonesia, Farmakope Herbal Indonesia Edisi I, 2008, Departemen Kesehatan Republik Indonesia, Jakarta Rafi, M., Rohaeti, E., Miftahudin, A., Darusman, L.K., Indones. heyneana, and Zingiber cassumunar was differentiated through R f 0.26 R f 0.17 and R f 0.10. The profile of fingerprint compound on Curcuma longa, C. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (R f 0.63), demethoxycurcumin (R f 0.34), bisdemethoxycurcumin (R f 0.21).
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Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF 254 as the stationary phase, chloroform:dichloromethane:methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent.
This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color.
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